منابع مشابه
Mechanism of regulation of hsp70 chaperones by DnaJ cochaperones.
Hsp70 chaperones assist a large variety of protein folding processes within the entire lifespan of proteins. Central to these activities is the regulation of Hsp70 by DnaJ cochaperones. DnaJ stimulates Hsp70 to hydrolyze ATP, a key step that closes its substrate-binding cavity and thus allows stable binding of substrate. We show that DnaJ stimulates ATP hydrolysis by Escherichia coli Hsp70, Dna...
متن کاملControl of the DnaK chaperone cycle by substoichiometric concentrations of the co-chaperones DnaJ and GrpE.
The polypeptide binding and release cycle of the molecular chaperone DnaK (Hsp70) of Escherichia coli is regulated by the two co-chaperones DnaJ and GrpE. Here, we show that the DnaJ-triggered conversion of DnaK.ATP (T state) to DnaK.ADP.Pi (R state), as monitored by intrinsic protein fluorescence, is monophasic and occurs simultaneously with ATP hydrolysis. This is in contrast with the T-->R c...
متن کاملInfluence of DnaK and DnaJ chaperones on Escherichia coli membrane lipid composition.
The content of fatty acids extracted from the membranes of E. coli MC 1061 harboring the wild-type dnaKdnaJ alleles and its delta dnaJ and delta dnaKdnaJ derivatives was compared. It was demonstrated that dodecanoic acid was missing in NPLs fraction extracted from both mutants grown at 42 degrees C. Phospholipids extracted from mutant strains were deprived of hexadecanoic acid methyl ester and ...
متن کاملWagner's canalization model.
Wagner (1996, Does evolutionary plasticity evolve? Evolution 50, 1008-1023.) and Siegal and Bergman, 2002 and Azevedo et al., 2006 have studied a simple model of the evolution of a network of N genes, in order to explain the observed phenomenon that systems evolve to be robust. These authors primarily considered the case N=10 and used simulations to reach their conclusions. Here we investigate ...
متن کاملPatients' attitudes to chaperones.
In a survey of 200 female patients attending a five-man practice in a health centre, 75 per cent of the respondents stated that they would like to be offered a chaperone at pelvic examinations. Only six per cent would accept the offer if the examination was performed by their own doctor and 17 per cent if a different doctor examined them. Patients expressing a definite wish for a chaperone were...
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ژورنال
عنوان ژورنال: Journal of Experimental Zoology Part A: Ecological and Integrative Physiology
سال: 2019
ISSN: 2471-5638
DOI: 10.1002/jez.2254